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利福平通过使DNA-RNA聚合酶-寡核苷酸复合物不稳定来抑制RNA合成。

Rifampicin inhibition of RNA synthesis by destabilisation of DNA-RNA polymerase-oligonucleotide-complexes.

作者信息

Schulz W, Zillig W

出版信息

Nucleic Acids Res. 1981 Dec 21;9(24):6889-906. doi: 10.1093/nar/9.24.6889.

DOI:10.1093/nar/9.24.6889
PMID:6174947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327649/
Abstract

Although the antibiotic rifampicin inhibits the transcription of poly[d(A-T)] by E.coli RNA polymerase, a series of short oligonucleotides is produced. It is claimed that the overall inhibition of RNA synthesis by rifampicin is caused by a destabilising effect on the binding of the intermediate oligonucleotides to the active enzyme-DNA complex. Rifampicin itself can only interact specifically with RNA polymerase if the enzyme is free or in a binary complex with DNA. However, the enzyme is not susceptible in a ternary complex, even if the "RNA" is as short as a trinucleotide.

摘要

尽管抗生素利福平会抑制大肠杆菌RNA聚合酶对聚[d(A-T)]的转录，但仍会产生一系列短寡核苷酸。据称，利福平对RNA合成的总体抑制是由于中间寡核苷酸与活性酶-DNA复合物结合的不稳定效应所致。只有当酶处于游离状态或与DNA形成二元复合物时，利福平本身才能与RNA聚合酶特异性相互作用。然而，即使“RNA”短至三核苷酸，该酶在三元复合物中也不易受影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/f653b4f3cbf2/nar00417-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/e9e84e66f8da/nar00417-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/0fa71be451f7/nar00417-0247-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/0809d8a502ae/nar00417-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/40fc14d73500/nar00417-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/f653b4f3cbf2/nar00417-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/e9e84e66f8da/nar00417-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/0fa71be451f7/nar00417-0247-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/0809d8a502ae/nar00417-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/40fc14d73500/nar00417-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416c/327649/f653b4f3cbf2/nar00417-0256-a.jpg

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