Chervenak R, Cohen J J
Thymus. 1982 Feb;4(2):61-7.
A series of experiments was undertaken to ascertain the reliability of peanut agglutinin (PNA) binding as a means of distinguishing mouse pre-T cells from other cell types. Normal bone marrow, thymus and spleen cells were stained with graded doses of fluoresceinated lectin (FITC-PNA) and subsequently examined by fluorescence microscopy to determine the number of lectin-binding cells. In addition, the PNA binding properties of mitogenically activated lymphocytes were examined in a similar manner. The results of this study show that PNA binding does not define a fixed population of cells, as the percentage of PNA+ cells detected in normal mouse tissues varied directly with the concentration of PNS used for staining. Nor is PNA able to distinguish between pre-T cells and mature T cells after mitogenic stimulation, both of which are shown to bind PNA. This lectin may , however, have value as a general marker of lymphoid precursors and activated lymphocytes of the T lineage.
进行了一系列实验以确定花生凝集素（PNA）结合作为区分小鼠前T细胞与其他细胞类型的方法的可靠性。用分级剂量的荧光素化凝集素（FITC-PNA）对正常骨髓、胸腺和脾细胞进行染色，随后通过荧光显微镜检查以确定凝集素结合细胞的数量。此外，以类似方式检查了有丝分裂原激活的淋巴细胞的PNA结合特性。这项研究的结果表明，PNA结合并不能定义一个固定的细胞群体，因为在正常小鼠组织中检测到的PNA+细胞百分比与用于染色的PNS浓度直接相关。有丝分裂原刺激后，PNA也无法区分前T细胞和成熟T细胞，这两种细胞均显示能结合PNA。然而，这种凝集素可能作为T系淋巴前体和活化淋巴细胞的一般标志物具有价值。
