Evans J N, Kelley J, Low R B, Adler K B
Am Rev Respir Dis. 1982 Jan;125(1):89-94. doi: 10.1164/arrd.1982.125.1.89.
The contractile capability of lung parenchymal strips isolated from normal rats was compared with that of strips isolated from rats with pulmonary fibrosis induced by intratracheal instillation of bleomycin sulfate. Subsequently, the population of contractile cells, both muscle and nonmuscle, was analyzed in each strip by histochemical, immunocytochemical, and ultrastructural means. The force (g force/g tissue wet weight) generated by fibrotic strips was approximately double that of the control strips in response to acetylcholine, epinephrine, and potassium depolarizing solution. The content of smooth muscle in fibrotic and control strips was not significantly different. Immunofluorescent examination indicated increased contractile proteins (actin and myosin) in the thickened alveolar walls of the fibrotic strips in areas devoid of histologically demonstrable smooth muscle. Ultrastructural examination of the fibrotic interstitium revealed an increased population of filament-laden cells that appeared related to the contractile interstitial cell, or myofibroblast. The results indicate that parenchymal strips isolated from fibrotic rat lung can generate increased force in vitro. These responses may be related to increased nonmuscle contractile cells in the fibrotic interstitium.
将从正常大鼠分离的肺实质条带的收缩能力与从经气管内滴注博来霉素诱导肺纤维化的大鼠分离的条带的收缩能力进行比较。随后，通过组织化学、免疫细胞化学和超微结构方法分析每条条带中收缩细胞（包括肌肉细胞和非肌肉细胞）的数量。在对乙酰胆碱、肾上腺素和钾去极化溶液的反应中，纤维化条带产生的力（克力/克组织湿重）约为对照条带的两倍。纤维化条带和平对照条带中平滑肌的含量没有显著差异。免疫荧光检查表明，在纤维化条带增厚的肺泡壁中，在无组织学可证实平滑肌的区域，收缩蛋白（肌动蛋白和肌球蛋白）增加。对纤维化间质的超微结构检查显示，充满细丝的细胞数量增加，这些细胞似乎与收缩性间质细胞或肌成纤维细胞有关。结果表明，从纤维化大鼠肺分离的实质条带在体外可产生更大的力。这些反应可能与纤维化间质中增加的非肌肉收缩细胞有关。
