Hoile R W
Ann R Coll Surg Engl. 1982 Mar;64(2):96-104.
The technique and validation of the cytochemical section bioassay for gastrin-like activity are described. This assay utilises the measurement of hormone-induced changes of carbonic anhydrase activity in guinea-pig gastric parietal cells. The clinical use of the assay as identified elevated levels of fasting gastrin-like biological activity in the plasma of patients with duodenal ulcer and has also demonstrated discrepancies between radioimmunoreactive gastrin levels, biological activity, and the clinical presentation of peptic ulcer disease.
本文描述了胃泌素样活性细胞化学切片生物测定法的技术和验证过程。该测定法利用豚鼠胃壁细胞中激素诱导的碳酸酐酶活性变化进行测量。该测定法的临床应用表明，十二指肠溃疡患者血浆中空腹胃泌素样生物活性水平升高，并且还显示出放射免疫反应性胃泌素水平、生物活性与消化性溃疡疾病临床表现之间存在差异。
