Eckersall P D, Beeley J A
Biochem Genet. 1981 Dec;19(11-12):1055-62. doi: 10.1007/BF00484564.
The isozymes of human salivary alpha-amylase have been separated by thin-layer gel isoelectric focusing in a pH 4-8 gradient followed by a starch-iodine zymogram procedure. The normal isozyme pattern (N) consisted of five major isozymes together with a number of minor ones. In addition, seven variant isozyme phenotypes were also observed, which had a combined frequency of 11.7% in a random population of 368 individuals. Analysis of familial data is indicative of the inheritance of autosomal codominant alleles.
人唾液α-淀粉酶的同工酶已通过在pH 4 - 8梯度下的薄层凝胶等电聚焦,随后进行淀粉-碘酶谱法分离。正常同工酶模式(N)由五种主要同工酶以及一些次要同工酶组成。此外,还观察到七种变异同工酶表型,在368名个体的随机群体中,其合并频率为11.7%。家族数据分析表明为常染色体共显性等位基因遗传。