Trojan J, Uriel J
Biomedicine. 1981 Dec;34(3):140-6.
Solid tumours with histopathological characteristics of teratocarcinoma (generally presenting the structures of nervous origin) were induced in the mouse by subcutaneous inoculation with murine embryonal carcinoma (EC) cells of line PCC4-azal. An indirect immunoperoxidase technique using anti-mouse AFP antibodies was applied for the localization of the protein on paraffin sections of tissue fixed with acetic acid-ethanol. The PCC4-azal EC cells were AFP negative in vitro. In the solid tumours, the immature embryonal or fetal components (i. e. tubes or cysts mimicking the development of the neural tube) and the groups of neuroepithelial cells arranged in more or less differentiating structures (pseudotubes, cluters) were AFP positive; on the contrary, cells of the same differentiated neuroepithelial type, but lacking recognizable tissue architecture, were AFP negative. Mature, adult components were AFP negative. Undifferentiated cancer components, whether structured or not, were AFP negative. These results suggest that the intracellular presence of AFP requires a minimum degree of both cell differentiation and tissue organization.
通过皮下接种PCC4-azal系小鼠胚胎癌（EC）细胞，在小鼠体内诱导出具有畸胎癌组织病理学特征（通常呈现神经源性结构）的实体瘤。使用抗小鼠甲胎蛋白（AFP）抗体的间接免疫过氧化物酶技术，对用醋酸-乙醇固定的组织石蜡切片上的该蛋白进行定位。PCC4-azal EC细胞在体外为AFP阴性。在实体瘤中，未成熟的胚胎或胎儿成分（即模仿神经管发育的管或囊肿）以及排列成或多或少分化结构（假管、团块）的神经上皮细胞群为AFP阳性；相反，相同分化神经上皮类型但缺乏可识别组织结构的细胞为AFP阴性。成熟的成年成分AFP阴性。未分化的癌成分，无论有无结构，均为AFP阴性。这些结果表明，AFP在细胞内的存在需要细胞分化和组织组织达到最低程度。
