Takano T, Takigawa M, Suzuki F
Med Biol. 1981 Dec;59(5-6):423-7.
Addition of putrescine to cultures of rabbit costal chondrocytes during the log-phase stimulated expression of their differentiated phenotype, as judged by increase in the synthesis of glycosaminoglycans and of metachromasia with toluidine blue staining. Putrescine stimulated glycosaminoglycan synthesis maximally at a concentration of 10(-7) M, and spermidine and spermine were also effective at similar concentration. These polyamines had little effect on DNA synthesis, RNA synthesis, DNA accumulation, or protein accumulation. These findings suggest that polyamines may be important in the expression of the differentiated phenotype of chondrocytes in culture.
在对数生长期向兔肋软骨细胞培养物中添加腐胺，可刺激其分化表型的表达，这可通过糖胺聚糖合成增加以及甲苯胺蓝染色的异染性增强来判断。腐胺在浓度为10(-7) M时对糖胺聚糖合成的刺激作用最大，亚精胺和精胺在相似浓度下也有效。这些多胺对DNA合成、RNA合成、DNA积累或蛋白质积累几乎没有影响。这些发现表明，多胺可能在培养的软骨细胞分化表型的表达中起重要作用。
