Takigawa M, Takano T, Shirai E, Suzuki F
Cell Differ. 1984 Aug;14(3):197-204. doi: 10.1016/0045-6039(84)90046-0.
Cytochalasin B changed the shape of cultured rabbit costal chondrocytes from polygonal to nearly spherical and stimulated glycosaminoglycan synthesis, which is a differentiated phenotype of chondrocytes, whereas colchicine changed them from polygonal to flattened and inhibited glycosaminoglycan synthesis. These morphological changes occurred parallel with the changes in glycosaminoglycan synthesis. Induction of ornithine decarboxylase by parathyroid hormone, which is a good marker of differentiated chondrocytes, was markedly potentiated in the spherical cells which had been pretreated with cytochalasin B, whereas pretreatment with colchicine inhibited the induction of the enzyme. Both cytochalasin B and colchicine inhibited DNA synthesis. The inhibitions were observed after the appearance of changes in the morphology of the cells and glycosaminoglycan synthesis. These findings suggest that intactness of microtubules and disruption of microfilaments are involved in regulating the expression of the differentiated phenotype of chondrocytes in culture.
细胞松弛素B使培养的兔肋软骨细胞的形状从多边形变为近球形，并刺激了糖胺聚糖的合成，而糖胺聚糖合成是软骨细胞的一种分化表型，而秋水仙碱则使细胞从多边形变为扁平形，并抑制了糖胺聚糖的合成。这些形态学变化与糖胺聚糖合成的变化同时发生。甲状旁腺激素对鸟氨酸脱羧酶的诱导作用（这是分化软骨细胞的一个良好标志物）在经细胞松弛素B预处理的球形细胞中明显增强，而秋水仙碱预处理则抑制了该酶的诱导。细胞松弛素B和秋水仙碱均抑制DNA合成。在细胞形态和糖胺聚糖合成出现变化后观察到了这种抑制作用。这些发现表明，微管的完整性和微丝的破坏参与调节培养软骨细胞分化表型的表达。
