Tabel H
Can J Comp Med. 1981 Jul;45(3):291-8.
Rabbits produced antibodies to a factor B-like serum protein (factor Bbov), its conversion product B gamma 2 and some other bovine serum proteins after repeated immunization with zymosan which previously had been incubated with fresh bovine serum. Such antisera were used to monitor purification of B gamma 2 from fresh bovine sera incubated with zxymosan. Subsequently, antisera specific for factor Bbov and B gamma 2 were produced. Antiserum produced against B gamma 2 cross-reacted with factor Bbov. Functional assays for factor Bbov were carried out in a hemolytic system with guinea pig erythrocytes in EGTA buffer. Heat inactivation (56 degrees C/5 min) of bovine serum destroyed the antigenicity of factor Bbov but not that of B gamma 2. Factor Bbov had an apparent molecular weight of 95,000 and B gamma 2 a molecular weight of 40,000 daltons. Conversion of factor Bbov to B gamma 2 was determined qualitatively by immunoelectrophoresis and quantitatively by radial immunodiffusion. Conversion of factor Bbov to B gamma 2 in bovine serum, in the presence of zymosan or cobra venom factor (CoVF) required Mg++ but not Ca++, did not occur in heat inactivated (56 degrees C/5 min) serum and was maximal, but not complete, when fresh bovine serum was incubated with zymosan (20 mg/mL) at 37 for two hours.
在用与新鲜牛血清孵育过的酵母聚糖反复免疫后，兔子产生了针对一种B因子样血清蛋白（B因子兔）、其转化产物Bγ2和其他一些牛血清蛋白的抗体。此类抗血清用于监测从与酵母聚糖孵育的新鲜牛血清中纯化Bγ2的过程。随后，制备了针对B因子兔和Bγ2的特异性抗血清。针对Bγ2产生的抗血清与B因子兔发生交叉反应。在含有豚鼠红细胞的EGTA缓冲液的溶血系统中对B因子兔进行功能测定。牛血清经热灭活（56℃/5分钟）后破坏了B因子兔的抗原性，但未破坏Bγ2的抗原性。B因子兔的表观分子量为95,000，Bγ2的分子量为40,000道尔顿。通过免疫电泳定性测定B因子兔向Bγ2的转化，并通过放射免疫扩散定量测定。在酵母聚糖或眼镜蛇毒因子（CoVF）存在的情况下，牛血清中B因子兔向Bγ2的转化需要Mg++而不是Ca++，在热灭活（56℃/5分钟）的血清中不发生，当新鲜牛血清与酵母聚糖（20mg/mL）在37℃孵育两小时时，转化达到最大值但不完全。
