Inada K
Jpn J Exp Med. 1980 Feb;50(1):13-21.
Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa was investigated. The ability of OEP to consume the hemolytic complement (CH50) of guinea pig serum (GPS) was relatively lower than zymosan, a potent activator of the alternative pathway (AP). The profiles of the complement consumption with OEP in GPS suggested that classical pathway (CP) activation seems to occur in addition to the AP-activation, because the consumption of the C3-C9, i.e., C3 total was relatively higher than those of C1, C4 and C2. Further results stated below is confirmed this. OEP consumed complement in certain extent in C4-deficient GPS and also in factor B- or D-depleted GPS. In these sera either pathway of the AP or the CP was operated. However, OEP consumed complement quite in full extent in the EGTA-chelated serum, in which AP-activation took place in normal level. OEP was therefore found in this experiment to activate only the AP. The conversion of C3 in immunoelectrophoresis which is a evidence of the CP-and/or AP-activation was observed with OEP. It was suggested that the antibody against OEP did not participate in the consumption of complement, because the GPS priorly absorbed with OEP-coated sheep RBC, was retained fully to react with OEP. It was discussed that the active site of OEP as to the complement activation located on the LPS portion of OEP.
对铜绿假单胞菌富含蛋白质的内毒素（OEP）的补体激活特性进行了研究。OEP消耗豚鼠血清（GPS）溶血补体（CH50）的能力相对低于酵母聚糖，酵母聚糖是替代途径（AP）的有效激活剂。GPS中OEP补体消耗情况表明，除AP激活外，经典途径（CP）似乎也被激活，因为C3 - C9（即总C3）的消耗相对高于C1、C4和C2。下文所述的进一步结果证实了这一点。OEP在C4缺陷的GPS以及B因子或D因子耗尽的GPS中在一定程度上消耗补体。在这些血清中，AP或CP的任何一条途径都起作用。然而，OEP在EGTA螯合血清中能充分消耗补体，在该血清中AP激活处于正常水平。因此，在本实验中发现OEP仅激活AP。在免疫电泳中观察到OEP导致C3转化，这是CP和/或AP激活的证据。有人提出抗OEP抗体不参与补体消耗，因为预先用包被OEP的绵羊红细胞吸附的GPS仍能与OEP充分反应。讨论了OEP关于补体激活的活性位点位于OEP的LPS部分。
