Fenton J, Foery R, Piatt L, Geschwindt K
Clin Chem. 1982 Apr;28(4 Pt 1):704-6.
We tested a new amylase reagent involving chromogenic substrates (Pantrak, Calbiochem-Behring) for precision, dynamic range, and susceptibility to potential interferences. Two p-nitrophenyl-alpha-maltaosides are used as substrates, which amylase cleaves to shorter-chain p-nitrophenyl maltaosides, the latter then yielding p-nitrophenol from the activity of alpha-glucosidase. A series of 100 specimens were tested by Pantrak and two established methodologies. In both cases, correlation was excellent. Precision was good for all methods; CVs for Pantrak were 3.0 to 4.5%. The dynamic range of Pantrak extended to 800 U/L. Above-normal quantities of triglyceride, hemoglobin, or bilirubin did not cause spurious results, but anticoagulants that bind divalent cations should not be used. The Pantrak method has desirable analytical features and is easy to use.
我们测试了一种涉及发色底物（Pantrak，Calbiochem - Behring）的新型淀粉酶试剂，以评估其精密度、动态范围以及对潜在干扰的敏感性。两种对硝基苯基 - α - 麦芽三糖苷用作底物，淀粉酶将其裂解为较短链的对硝基苯基麦芽三糖苷，后者再通过α - 葡萄糖苷酶的活性产生对硝基苯酚。通过Pantrak和两种既定方法对一系列100个标本进行了检测。在这两种情况下，相关性都非常好。所有方法的精密度都很好；Pantrak的变异系数为3.0%至4.5%。Pantrak的动态范围扩展到800 U/L。甘油三酯、血红蛋白或胆红素含量高于正常水平不会导致假结果，但不应使用结合二价阳离子的抗凝剂。Pantrak方法具有理想的分析特性且易于使用。
