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关于在培养细胞的流式细胞术分析中使用淋巴细胞作为标准的注意事项。

Caution on the use of lymphocytes as standards in the flow cytometric analysis of cultured cells.

作者信息

Wolley R C, Herz F, Koss L G

出版信息

Cytometry. 1982 May;2(6):370-3. doi: 10.1002/cyto.990020603.

DOI:10.1002/cyto.990020603
PMID:6176406
Abstract

Flow cytometry was used to compare the fluorescence intensity of the G0/G1 peak of a diploid (WI-38), a hypodiploid (C4I) and a heteroploid (KB) human cell line with that of nonstimulated human lymphocytes. With all three cultured cells the fluorescence intensity was substantially higher than that of lymphocytes. These results, obtained with three different DNA-intercalating fluorochromes, suggest that lymphocytes may not be the standard of choice for flow cytometric studies of the DNA distribution in human cell cultures and that diploid cells such as WI-38 may be more appropriate for this purpose.

摘要

运用流式细胞术比较二倍体（WI - 38）、亚二倍体（C4I）和异倍体（KB）人细胞系G0/G1峰的荧光强度与未受刺激的人淋巴细胞的荧光强度。对于所有这三种培养细胞，其荧光强度均显著高于淋巴细胞。用三种不同的DNA嵌入荧光染料获得的这些结果表明，淋巴细胞可能并非用于流式细胞术研究人细胞培养物中DNA分布的首选标准，而诸如WI - 38这样的二倍体细胞可能更适合此目的。

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