Givan A L, Shenton B K, Carr T W
Department of Surgery, University of Newcastle upon Tyne, England.
Cytometry. 1988 May;9(3):271-4. doi: 10.1002/cyto.990090314.
Flow cytometric measurements of nuclear DNA content often involve the calculation of a DNA index, which compares the DNA fluorescence from two different populations. Such DNA indices have been used to classify aneuploid peaks from tumour tissue into different categories relative to normal diploid cells. This report describes a correction based on the channel for unstained particles that is required if DNA index values are to give a true and reproducible indication of relative DNA content.
细胞核DNA含量的流式细胞术测量通常涉及DNA指数的计算，该指数比较来自两个不同群体的DNA荧光。此类DNA指数已被用于将肿瘤组织中的非整倍体峰相对于正常二倍体细胞分类到不同类别中。本报告描述了一种基于未染色颗粒通道的校正方法，若要使DNA指数值能够真实且可重复地指示相对DNA含量，则需要进行这种校正。
