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从铝佐剂疫苗中洗脱的口蹄疫病毒抗原的定量、表征及安全性测试

Quantification, characterisation and safety testing of foot-and-mouth disease virus antigens eluted from alhydrogel vaccines.

作者信息

Visser N, Woortmeyer R

出版信息

Dev Biol Stand. 1981;50:277-83.

PMID:6176487
Abstract

Different methods for the elution and the concentration of FMDV-antigen from formaldehyde-inactivated alhydrogel vaccines were compared. With locally produced vaccines the best results were obtained if the antigen was eluted with potassium phosphate buffer and the concentration was performed by ultrafiltration. Final recoveries of 50% of the 140-S antigen could be obtained. For innocuity control 1-3 mg of 140-S antigen was eluted from 21 vaccine. SDS-polyacrylamide gel electrophoresis and iso-electric focusing patterns of eluted antigen will be discussed.

摘要

比较了从甲醛灭活的铝胶疫苗中洗脱和浓缩口蹄疫病毒抗原的不同方法。对于本地生产的疫苗，如果用磷酸钾缓冲液洗脱抗原并通过超滤进行浓缩，可获得最佳结果。140-S抗原的最终回收率可达50%。为进行无害化控制，从21支疫苗中洗脱了1-3毫克的140-S抗原。将讨论洗脱抗原的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和等电聚焦图谱。

相似文献

1
Quantification, characterisation and safety testing of foot-and-mouth disease virus antigens eluted from alhydrogel vaccines.从铝佐剂疫苗中洗脱的口蹄疫病毒抗原的定量、表征及安全性测试
Dev Biol Stand. 1981;50:277-83.
2
Conditions for proper formaldehyde inactivation of foot and mouse disease alhydrogel vaccines.
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Innocuity testing of foot-and-mouth disease vaccines. I. Formaldehyde-inactivated alhydrogel vaccines.口蹄疫疫苗的无害性检测。I. 甲醛灭活氢氧化铝凝胶疫苗
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Attempts at potency testing of foot-and-mouth disease vaccines by evaluation of the complement fixing capacity of eluates.通过评估洗脱液的补体结合能力对口蹄疫疫苗进行效力测试的尝试。
Dev Biol Stand. 1976;35:297-300.
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[Methods of testing of safety of the anti-foot-and-mouth disease vaccine].[口蹄疫疫苗安全性检测方法]
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[Possibilities of biophysicochemical antigen control in vaccine production using foot-and-mouth disease virus as an example (review)].[以口蹄疫病毒为例探讨疫苗生产中生物物理化学抗原控制的可能性（综述）]
Arch Exp Veterinarmed. 1988 Mar;42(2):165-73.
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[Comparative testing of the quality of foot-and-mouth disease vaccines prepared with different virus inactivators].[不同病毒灭活剂制备的口蹄疫疫苗质量的比较试验]
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[Safety of foot-and-mouth disease vaccines in cell cultures].[细胞培养中口蹄疫疫苗的安全性]
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Innocuity testing of foot-and-mouth disease vaccines. II. Aziridine-inactivated antigen produced in baby hamster kidney cells.
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Preparation of virus-infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus from inactivated vaccine.从灭活疫苗中制备口蹄疫病毒感染相关（VIA）抗原
J Vet Med Sci. 1996 Jun;58(6):599-601. doi: 10.1292/jvms.58.599.

引用本文的文献

1
Formaldehyde inactivation of foot-and-mouth disease virus. Conditions for the preparation of safe vaccine.口蹄疫病毒的甲醛灭活。安全疫苗制备条件。
Arch Virol. 1984;80(2-3):103-17. doi: 10.1007/BF01310652.