Májský A
Folia Haematol Int Mag Klin Morphol Blutforsch. 1981;108(6):745-51.
The Ferrone's hypothesis elucidating the effect of rabbit complement in HLA typing using the cytotoxic test, due to the presence of xenocytotoxins to human lymphocytes in the rabbit serum was corroborated by the following experiments: 1. In human serum active as complement in HLA typing non-HLA lymphocytotoxins with optimum activity at 20 degree C were shown. In the guinea pig complement ineffective in HLA typing no cytotoxin to human lymphocytes were found. 2. In the rabbit complement cytotoxins to peripheral human lymphocytes were found when the incubation period of the cytotoxic test was prolonged to 3-4 h. 3. During the cytotoxic test on a model of rabbit immune sera - rabbit lymphocytes human complement showed a substantially higher activity than the rabbit complement. In the human complement xenocytotoxins to rabbit lymphocytes were demonstrated.
费罗内关于兔补体在使用细胞毒性试验进行HLA分型中的作用的假说，由于兔血清中存在针对人淋巴细胞的异种细胞毒素而得到了以下实验的证实：1. 在HLA分型中作为补体起作用的人血清中，发现了在20℃时具有最佳活性的非HLA淋巴细胞毒素。在HLA分型中无效的豚鼠补体中，未发现针对人淋巴细胞的细胞毒素。2. 当细胞毒性试验的孵育期延长至3 - 4小时时，在兔补体中发现了针对外周人淋巴细胞的细胞毒素。3. 在兔免疫血清 - 兔淋巴细胞 - 人补体模型的细胞毒性试验中，人补体显示出比兔补体高得多的活性。在人补体中证实了针对兔淋巴细胞的异种细胞毒素。
