Májský A
Vnitr Lek. 1989 Nov;35(11):1102-5.
The use of human complement in HLA typing produced strongly positive results only in examinations with polyspecific sera from pregnant women, while with mono- or bispecific sera positive results were obtained only in some instances and the results were usually weaker. With polyspecific sera from patients after multiple transfusions negative results were obtained. The action of complement did not depend on the titre of HLA cytotoxins. The results confirm the hitherto held view that it is not suitable to use human complement for HLA typing.
在HLA分型中使用人补体，仅在使用孕妇多特异性血清进行检测时产生强阳性结果，而使用单特异性或双特异性血清时，仅在某些情况下获得阳性结果，且结果通常较弱。在多次输血患者的多特异性血清检测中获得阴性结果。补体的作用不取决于HLA细胞毒素的滴度。这些结果证实了迄今为止一直持有的观点，即用人补体进行HLA分型是不合适的。
