Torrens Y, Michelot R, Beaujouan J C, Glowinski J, Bockaert J
J Neurochem. 1982 Jun;38(6):1728-34. doi: 10.1111/j.1471-4159.1982.tb06655.x.
Rats with a push-pull cannula implanted in the right striatum were used to study the biosynthesis of 35S-substance P (SP) from [35S]methionine and its transport to the ipsilateral substantia nigra. [35S]Methionine was delivered for 2, 3 or 5 h to the push-pull cannula. 35S-SP in striatal and nigral tissues was estimated after immunoadsorption and HPLC. Higher levels of 35S-SP in striatal homogenates were found after a 5-h labelling period. 35S-P biosynthesis was inhibited when cycloheximide was superfused together with [35S]methionine. The identity of 35S-SP was further checked by its conversion into 35S-SP sulphoxide. After a 5-h labelling period, 35S-SP was also recovered in the substantia nigra. This was not the case after hemisection of striato-nigral fibers. When rats were killed 15 or 24 h after the 5-h labelling period, 35S-SP levels in the substantia nigra were higher than those found just after 5-h labelling period, while the reverse was observed in the striatum.
将推挽式套管植入右侧纹状体的大鼠用于研究[35S]甲硫氨酸合成35S-物质P（SP）及其向同侧黑质的转运。将[35S]甲硫氨酸输送至推挽式套管2、3或5小时。免疫吸附和高效液相色谱法（HPLC）后估计纹状体和黑质组织中的35S-SP水平。5小时标记期后，纹状体匀浆中35S-SP水平更高。当环己酰亚胺与[35S]甲硫氨酸一起灌流时，35S-P的生物合成受到抑制。通过将35S-SP转化为35S-SP亚砜进一步检查35S-SP的同一性。5小时标记期后，黑质中也回收了35S-SP。纹状体-黑质纤维半切后情况并非如此。在5小时标记期后15或24小时处死大鼠时，黑质中35S-SP水平高于5小时标记期刚结束时发现的水平，而在纹状体中观察到相反情况。
