Bannon M J, Goedert M
Brain Res. 1984 May 28;301(1):184-6. doi: 10.1016/0006-8993(84)90421-9.
In all but one brain region sampled, substance P-like immunoreactivity (SPLI) was unchanged 4 h after a dose of cycloheximide that produced a near-total inhibition of rat brain protein synthesis. This suggests that brain substance P (SP) utilization is slow under basal conditions. The administration of the dopamine antagonist haloperidol to cycloheximide-pretreated rats apparently accelerated striatonigral SP utilization, as evidenced by large depletions in SPLI in the substantia nigra and striatum. Measuring the decline of SPLI a short time after protein synthesis inhibition may provide an index of brain SP utilization.
在除一个采样脑区外的所有脑区中，给予能几乎完全抑制大鼠脑蛋白质合成的环己酰亚胺剂量4小时后，P物质样免疫反应性（SPLI）未发生变化。这表明在基础条件下脑P物质（SP）的利用较为缓慢。向经环己酰亚胺预处理的大鼠施用多巴胺拮抗剂氟哌啶醇显然加速了纹状体黑质SP的利用，黑质和纹状体中SPLI的大量减少证明了这一点。在蛋白质合成抑制后短时间内测量SPLI的下降可能提供脑SP利用的一个指标。
