Specificities of human heterophilic Hanganutziu and Deicher (H-D) antibodies and avian antisera against H-D antigen-active glycosphingolipids.

The precise specificities H-D antibodies and newly obtained avian antisera to the H-D antigen-active glycosphingolipids. N-glycolylneuraminyl (alpha 2-3) lactosyl ceramide (hematoside) and N-glycolylneuraminyl (alpha 2-3) lactoneotetraosyl ceramide (sialosylparagloboside) were compared by gel precipitation and hemagglutination inhibition using other gangliosides with a related structure and chemically modified hematoside derivatives and the oligosaccharides from both the antigenic glycosphingolipids. Human H-D antibodies were found to have the same affinity for both antigenic glycosphingolipids; however, the avian anti-hematoside or anti-sialosylparaglobosides showed a higher affinity for the homologous antigen. Human H-D antibodies were directed more exactly toward the terminal sialic acid residue and more roughly to the hydrophobic portion of both compounds; however, the two avian antibodies were directed more specificity to the whole structure of the homologous antigen glycosphingolipid.