Thompson S A, Johnson M P, Brooks C L
Prostate. 1982;3(1):45-58. doi: 10.1002/pros.2990030108.
A radioligand assay and the soluble peroxidase-antiperoxidase (PAP) technique were used to compare ovine and rat prolactin as ligands for the demonstration of prolactin binding sites in rat ventral, lateral, and dorsal prostate lobes. Biochemical techniques were effective in demonstrating prolactin binding sites in ventral prostate with both ovine and rat prolactin; however, binding sites could not be characterized in lateral or dorsal lobes with either ligand. Using the PAP technique, rat prolactin consistently produced a discrete, dense reaction product over binding sites in the Golgi zone of the ventral prostate; ovine prolactin produced a more dispersed pattern of staining throughout the entire supranuclear region of the cells. In ventral prostate, most epithelial cells were immunoreactive when either ligand was used. However, lateral prostate contained fewer densely immunoreactive cells than did ventral prostate, and rat prolactin was more effective in demonstrating these binding sites than was ovine prolactin. Dorsal prostate epithelium was highly immunoreactive for binding of both ligands; however, there was considerably more staining in this lobe that remained after absorption of the antibody with prolactin. These data suggest that the inability to demonstrate prolactin binding sites in the lateral lobe using radioligand assay may have been due to the fewer numbers of binding sites that were diluted below the sensitivity of the assay when a tissue homogenate preparation was used. Noncompetitive binding in the dorsal lobe, as seen in the radioligand assay, coincided with immunocytochemical findings of relatively high nonabsorbable background staining.
采用放射性配体测定法和可溶性过氧化物酶-抗过氧化物酶（PAP）技术，比较绵羊催乳素和大鼠催乳素作为配体，用于在大鼠腹侧、外侧和背侧前列腺叶中显示催乳素结合位点。生化技术能够有效地显示腹侧前列腺中绵羊催乳素和大鼠催乳素的结合位点；然而，两种配体均无法在外侧叶或背侧叶中鉴定出结合位点。使用PAP技术，大鼠催乳素始终在腹侧前列腺高尔基体区域的结合位点上产生离散、密集的反应产物；绵羊催乳素在细胞整个核上区域产生更分散的染色模式。在腹侧前列腺中，使用任何一种配体时，大多数上皮细胞都具有免疫反应性。然而，外侧前列腺中密集免疫反应性细胞比腹侧前列腺少，并且大鼠催乳素在显示这些结合位点方面比绵羊催乳素更有效。背侧前列腺上皮对两种配体的结合均具有高度免疫反应性；然而，用催乳素吸收抗体后，该叶中仍有相当多的染色。这些数据表明，使用放射性配体测定法无法在外侧叶中显示催乳素结合位点，可能是由于结合位点数量较少，当使用组织匀浆制剂时被稀释到测定灵敏度以下。如放射性配体测定中所见，背侧叶中的非竞争性结合与相对较高的不可吸收背景染色的免疫细胞化学结果一致。
