Witorsch R J
Prostate. 1982;3(5):459-73. doi: 10.1002/pros.2990030505.
Prolactin binding sites of ventral, lateral, and dorsal lobes of rat prostate were examined immunohistochemically 1, 2, 4, and 8 days after castration or sham operation. In sham-operated rats each lobe exhibited a distinct pattern of intracellular and intraluminal prolactin binding. A loss in prolactin binding from epithelial cells of ventral prostate, which was visualized postcastration, was quantitated with the aid of an image analyzer and then statistically evaluated. The proportion of ventral prostate epithelial cells devoid of prolactin binding increased from approximately 13% in sham-operated rats to approximately 29% 1 day after castration, and reached a peak level of about 71% 4 days postcastration. No loss of prolactin binding was evident in either lateral or dorsal prostate up to 8 days postcastration. Direct measurement of epithelial cell heights and subsequent statistical evaluation revealed similar regional differences in the rates and extent of prostate involution. Eight days after castration ventral prostate epithelial cell heights decreased by 56% whereas lateral and dorsal lobe epithelial cells heights decreased about 25% and 14%, respectively. The apparent relationship between testicular dependence of prostatic prolactin binding and castration-induced prostatic involution are discussed in terms of possible regional variations in the prolactin-androgen interplay in prostate.
在去势或假手术1、2、4和8天后，采用免疫组织化学方法检测大鼠前列腺腹叶、侧叶和背叶的催乳素结合位点。在假手术大鼠中，每个叶都呈现出细胞内和管腔内催乳素结合的独特模式。去势后可见腹侧前列腺上皮细胞催乳素结合减少，借助图像分析仪对其进行定量，然后进行统计学评估。无催乳素结合的腹侧前列腺上皮细胞比例从假手术大鼠中的约13%增加到去势后1天的约29%，并在去势后4天达到约71%的峰值水平。直至去势后8天，侧叶或背叶均未出现明显的催乳素结合丧失。对上皮细胞高度进行直接测量并随后进行统计学评估，结果显示前列腺 involution 的速率和程度存在类似的区域差异。去势8天后，腹侧前列腺上皮细胞高度下降了56%，而侧叶和背叶上皮细胞高度分别下降了约25%和14%。从前列腺中催乳素 - 雄激素相互作用可能存在的区域差异方面，讨论了前列腺催乳素结合对睾丸的依赖性与去势诱导的前列腺 involution 之间的明显关系。 （注：原文中“involution”未明确中文释义，可根据上下文灵活理解，这里暂保留英文）
