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单价A型葡萄球菌肠毒素抗血清的制备及其在葡萄球菌肠毒素分型中的应用

[Production of a monovalent staphylococcal enterotoxic antiserum type A and its use for typing staphylococcal enterotoxins].

作者信息

Fluer F S, Rybasova G V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1982 Apr(4):107-10.

PMID:6177130
Abstract

A monovalent specific staphylococcal antiserum, type A, was obtained by means of the isolated and purified preparation of type A staphylococcal enterotoxin. This antiserum was proved to be identical to antiserum of the same type, manufactured by Serva Feinbiochemica GmbH & Co. (West Germany). The titer of the newly obtained antiserum in the precipitation test was 1 : 16, and its use allowed one to detect enterotoxin of the above-mentioned type at a concentration of 0.004 mg/ml. The study of 320 staphylococcal strains with the use of this antiserum revealed that 25 strains (7.8%) produced type A enterotoxin.

摘要

通过分离纯化A型葡萄球菌肠毒素制备出了单价特异性A型葡萄球菌抗血清。经证明,这种抗血清与德国Serva Feinbiochemica GmbH & Co.公司生产的同型抗血清相同。新获得的抗血清在沉淀试验中的效价为1:16,使用该抗血清能够检测到浓度为0.004 mg/ml的上述类型的肠毒素。使用这种抗血清对320株葡萄球菌菌株进行研究发现,有25株(7.8%)产生A型肠毒素。

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