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培养细胞和红细胞表面干扰素受体的超微结构定位

Ultrastructural localization of interferon receptors on the surfaces of cultured cells and erythrocytes.

作者信息

Kushnaryov V M, Sedmak J J, Bendler J W, Grossberg S E

出版信息

Infect Immun. 1982 May;36(2):811-21. doi: 10.1128/iai.36.2.811-821.1982.

DOI:10.1128/iai.36.2.811-821.1982
PMID:6177639
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC351301/
Abstract

The bindings sites for interferon (IFN) on the limiting cell membranes of human and mouse fibroblasts and erythrocytes were revealed by an indirect immunoferritin technique. Mouse IFN-beta and human IFN-beta of high specific activity were used with the corresponding purified antibodies. Species-specific IFN binding was demonstrated by ferritin deposition on human erythrocytes and fibroblast membranes treated with human IFN and on mouse erythrocytes and fibroblast membranes treated with mouse IFN, but not on human erythrocytes or fibroblast membranes treated with mouse IFN. IFN binding sites on fibroblasts were located on regions of membranes between microvilli, whereas diphtheria toxin receptors were demonstrated mainly on microvilli. IFN binding altered the diphtheria toxin after IFN treatment. This reduced toxicity correlated with a decrease in the quantity of receptors for diphtheria toxin on the cell membrane. Thus, the species-specific binding of IFN appears to depend on membrane receptors in discrete regions of the limiting membrane which are present not only on functionally responsive fibroblasts but also on erythrocytes.

摘要

采用间接免疫铁蛋白技术揭示了干扰素（IFN）在人和小鼠成纤维细胞及红细胞的限制细胞膜上的结合位点。使用了具有高比活性的小鼠IFN-β和人IFN-β以及相应的纯化抗体。通过铁蛋白沉积在用人IFN处理的人红细胞和成纤维细胞膜以及用小鼠IFN处理的小鼠红细胞和成纤维细胞膜上，而不在用小鼠IFN处理的人红细胞或成纤维细胞膜上，证明了种属特异性IFN结合。成纤维细胞上的IFN结合位点位于微绒毛之间的膜区域，而白喉毒素受体主要在微绒毛上被证实。IFN处理后，IFN结合改变了白喉毒素。这种毒性降低与细胞膜上白喉毒素受体数量的减少相关。因此，IFN的种属特异性结合似乎取决于限制膜离散区域中的膜受体，这些受体不仅存在于功能反应性成纤维细胞上，也存在于红细胞上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/eb5a7631d316/iai00152-0386-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/e8308e082ca5/iai00152-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/d0784c618d52/iai00152-0382-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/ccc9398964ca/iai00152-0382-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/08242b4879d0/iai00152-0383-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/53256be0974a/iai00152-0384-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/179987701d05/iai00152-0385-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/eb5a7631d316/iai00152-0386-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/e8308e082ca5/iai00152-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/d0784c618d52/iai00152-0382-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/ccc9398964ca/iai00152-0382-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/08242b4879d0/iai00152-0383-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/53256be0974a/iai00152-0384-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/179987701d05/iai00152-0385-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b49c/351301/eb5a7631d316/iai00152-0386-a.jpg

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