Brunswick M, Sainis K B, Bromberg J S, Lake P
J Immunol. 1982 Aug;129(2):698-703.
The contact-sensitizing haptens dinitrophenyl (DNP) and oxazalone (Ox) act as helper determinants for antibody responses to Thy-1 when conjugated to donor thymus cells. The helper effect is transferrable from primed to naive mice with spleen cells, producing specific augmentation of in vivo PFC responses to Thy-1. The helper cells are hapten-specific and require associative recognition of hapten and Thy-1, excluding a role for nonspecific B cell activation. The phenotype of the helper cells is Thy-1+ and Lyt-1+2-. Antigen-specific suppression could be readily generated by using an inoculum of DNP-modified syngeneic RBC. T cells from these suppressed donors (Ts) were shown to abolish the helper effects of TH in adoptive transfer experiments in vivo. These Ts were characterized as Thy-1+ and Lyt-1-2+. A requirement for MHC compatibility at the I-J subregion was necessary between the Ts and the recipient to obtain a transfer of suppression.
接触致敏半抗原二硝基苯基（DNP）和恶唑酮（Ox）与供体胸腺细胞结合时，作为对Thy-1抗体应答的辅助决定簇。辅助效应可通过脾细胞从致敏小鼠转移至未致敏小鼠，使体内对Thy-1的PFC应答产生特异性增强。辅助细胞具有半抗原特异性，需要半抗原和Thy-1的联合识别，排除了非特异性B细胞活化的作用。辅助细胞的表型为Thy-1+和Lyt-1+2-。通过使用DNP修饰的同基因红细胞接种物可容易地产生抗原特异性抑制。在体内过继转移实验中，来自这些受抑制供体的T细胞（Ts）可消除TH的辅助效应。这些Ts的特征为Thy-1+和Lyt-1-2+。Ts与受体之间在I-J亚区需要MHC相容性才能实现抑制的转移。
