Garson J A, Beverley P C, Coakham H B, Harper E I
Nature. 1982 Jul 22;298(5872):375-7. doi: 10.1038/298375a0.
Conventional antibodies have long been used in an attempt to produce specific neural markers. Such markers would be invaluable for studying the structural organization and development of the nervous system. Unfortunately, they have not been found to discriminate satisfactorily between neuronal subpopulations. Recent developments of the hybridoma technique, however, promise to provide monoclonal antibodies of adequate specificity. Such antibodies have already generated and shown to be capable of distinguishing between individual neurones of the leech nervous system. We report here two monoclonal antibodies which, although generated against human T cells, react exclusively with Purkinje cells in the vertebrate central nervous system (CNS). This new specificity arose out of a fortuitous observation made during examination of the lymphocyte infiltration of human cerebellar tumours with the monoclonal antibody, UCHT1. Although widely used as a T-cell marker, its reaction with neural tissue has not hitherto been described. To our knowledge, this is the first description of a monoclonal antibody which recognises discrete neuronal population in the vertebrate brain.
传统抗体长期以来一直被用于尝试产生特定的神经标志物。这样的标志物对于研究神经系统的结构组织和发育将是非常宝贵的。不幸的是，尚未发现它们能够令人满意地区分神经元亚群。然而，杂交瘤技术的最新进展有望提供具有足够特异性的单克隆抗体。这样的抗体已经产生，并已证明能够区分水蛭神经系统中的单个神经元。我们在此报告两种单克隆抗体，它们虽然是针对人T细胞产生的，但仅与脊椎动物中枢神经系统（CNS）中的浦肯野细胞发生反应。这种新的特异性源于在用单克隆抗体UCHT1检查人类小脑肿瘤的淋巴细胞浸润过程中偶然观察到的结果。尽管UCHT1被广泛用作T细胞标志物，但迄今为止尚未描述其与神经组织的反应。据我们所知，这是首次描述一种识别脊椎动物脑中离散神经元群体的单克隆抗体。
