Sommer I, Schachner M
Neurosci Lett. 1982 Apr 16;29(2):183-8. doi: 10.1016/0304-3940(82)90351-2.
In freshly dissociated viable cells of early postnatal mouse cerebellum, O1 antigen-positive oligodendrocytes have been eliminated by complement dependent immunocytolysis. Before seeding residual cells in culture, O4 antigen-positive cells were immunolabeled by O4 antibody which had been directly conjugated with rhodamine. After various periods of time in culture, cells were treated with fluorescein conjugated O1 antibody, in order to assay for the appearance of O1-positive oligodendrocytes. After 6.5 h in vitro, the first cells carrying both the previously applied rhodamine label and the freshly applied fluorescein label were seen. The simultaneous appearance of both labels indicates tha O4-positive and O1-negative cells are direct precursors of O1 antigen-positive oligodendrocytes.
在新生小鼠小脑早期的新鲜解离活细胞中，O1抗原阳性少突胶质细胞已通过补体依赖性免疫细胞溶解被清除。在将残留细胞接种到培养物中之前，用直接与罗丹明偶联的O4抗体对O4抗原阳性细胞进行免疫标记。在培养不同时间段后，用荧光素偶联的O1抗体处理细胞，以检测O1阳性少突胶质细胞的出现情况。体外培养6.5小时后，首次观察到同时带有先前应用的罗丹明标记和新应用的荧光素标记的细胞。两种标记的同时出现表明O4阳性和O1阴性细胞是O1抗原阳性少突胶质细胞的直接前体。
