Sajjad S M, Ordóñez N G
Arch Pathol Lab Med. 1982 Jun;106(6):298-9.
A modified trichrome stain was used to detect hepatitis B surface antigen (HBsAg) in liver tissue obtained at the time of autopsy. The infected hepatocytes exhibited a selective green metachromasia of their cytoplasma, corresponding with the intracytoplasmic presence of HBsAg as confirmed by immunofluorescent and immunoperoxidase techniques. The trichrome technique for staining HBsAg is easy to perform, reliable, and can be used for routine histologic screening of HBsAg in liver disease.
采用改良三色染色法检测尸检时获取的肝组织中的乙型肝炎表面抗原（HBsAg）。受感染的肝细胞胞质呈现选择性绿色异染性，这与免疫荧光和免疫过氧化物酶技术证实的HBsAg胞质内存在情况相符。用于HBsAg染色的三色技术操作简便、可靠，可用于肝病中HBsAg的常规组织学筛查。
