Lambe D W, Ferguson K P, Mayberry W R
Can J Microbiol. 1982 Apr;28(4):367-74. doi: 10.1139/m82-056.
Bacteroides gingivalis is a newly proposed species which includes strains isolated from the mouth. Thirteen strains of B. gingivalis isolated from three geographic locations in the United States and France were examined with direct fluorescent antibody staining and analysis of total cellular fatty acids and compared with 16 strains of B. asaccharolyticus of nonoral origin by the same methods. Bacteroides gingivalis strains reacted with the B. gingivalis conjugate (fluorescein isothiocyanate labeled antibody reagent) only, while the B, asaccharolyticus strains reacted with the B. asaccharolyticus conjugate only. The B. gingivalis strains showed negative fluorescence with fluorescein isothiocyanate conjugates for other black-pigmented Bacteroides species. The specificity of the B. gingivalis conjugate was demonstrated by its failure to stain 88 strains of aerobic and anaerobic bacteria other than B. gingivalis. The fatty acid profiles of B. gingivalis and B. asaccharolyticus were readily distinguishable. The B. gingivalis profile was also distinguishable from those of other pigmenting Bacteroides species on the basis of concentration ratios among the characteristic components. These results support the species separation of B. gingivalis and B. asaccharolyticus. Further, they indicate the usefulness of cellular fatty acid profiles as an adjunct to the use of specific fluorescent antibody conjugates for identification of Bacteroides species.
牙龈拟杆菌是一个新提出的菌种，包括从口腔分离出的菌株。对从美国和法国三个地理位置分离出的13株牙龈拟杆菌进行了直接荧光抗体染色和全细胞脂肪酸分析，并通过相同方法与16株非口腔来源的解糖拟杆菌进行了比较。牙龈拟杆菌菌株仅与牙龈拟杆菌结合物（异硫氰酸荧光素标记抗体试剂）发生反应，而解糖拟杆菌菌株仅与解糖拟杆菌结合物发生反应。牙龈拟杆菌菌株对其他产黑色素拟杆菌种的异硫氰酸荧光素结合物呈阴性荧光。牙龈拟杆菌结合物的特异性通过其未能对除牙龈拟杆菌外的88株需氧菌和厌氧菌进行染色得以证明。牙龈拟杆菌和解糖拟杆菌的脂肪酸谱很容易区分。基于特征成分之间的浓度比，牙龈拟杆菌的谱也与其他产色素拟杆菌种的谱不同。这些结果支持牙龈拟杆菌和解糖拟杆菌的菌种分离。此外，它们表明细胞脂肪酸谱作为使用特异性荧光抗体结合物鉴定拟杆菌种的辅助手段是有用的。
