尿5-羟色胺酸的比色测定法。
Colorimetric assay of urinary 5-hydroxy-3-indoleacetic acid.
作者信息
Shihabi Z K, Wilson E L
出版信息
Clin Biochem. 1982 Apr;15(2):106-8. doi: 10.1016/s0009-9120(82)96125-2.
PMID:6178531
Abstract
We describe a method for 5-hydroxy-3-indoleacetic acid in urine based on its reaction with nitrosonaphthol in the presence of mercaptoethanolamine, an odorless compound. Mercaptoethanolamine shifts the color maximum from 540 nm to 640 nm, intensifies the color and discharges the color of interfering substances. The method is rapid and free from interferences.
摘要
我们描述了一种基于5-羟基-3-吲哚乙酸在无臭化合物巯基乙醇胺存在下与亚硝基萘酚反应的尿液中5-羟基-3-吲哚乙酸的检测方法。巯基乙醇胺将颜色最大值从540nm移至640nm，加深颜色并消除干扰物质的颜色。该方法快速且无干扰。
Zotero 插件