Awasthi V B, Singh U V
Gegenbaurs Morphol Jahrb. 1982;128(1):100-6.
The neurosecretory cells (NSCs) of the brain of the mature larvae and 3 months old diapause pupae of Amsacta collaris Hampson (Lepidopera: arctiidae) have been studied under different temperature regimes (0, 7, 18, 37, and 42 degrees C), using paraldehyde fuchsin and performic acid resorcin fuchsin staining techniques. The 0 degree C is fatal to the larvae, and at 7 degrees C, within 24 h the quantity of NSM reduced considerably in all the subtypes of A-cells; activity started 3rd day onwards, and on 5th day, the A1-cells appeared loaded with NSM, the A2-cells moderately filled, but no change in A3-cells was observed. At 0 degree C, within 48 h or chilling, the NSCs of diapause pupae (DP) revealed a moderate amount of NSM with numerous, large vacuoles in the A1-cells and in some insects, the cells appeared gigantic in form; the A2- and A3-cells were moderately filled with NSM. After 1 week of chilling, at 0 degree C, the A1-cells appeared almost empty. The A1-cells of DP-kept at 7 degrees C and 18 degrees C respectively, discharged quickly all the previously stored NSM, within 2 days, hence appeared poorly filled. 3rd day onwards, a gradual increase in NSM was observed. After 15 days, a moderate amount of NSM in A1-, a large amount in A3-, and increased amount in A2-cells of the medial and lateral groups was observed. The 37 degrees C appeared to be the most suitable temperature at which all the cells show maximum activity in mature larvae and DP both, and contain a moderate amount of NSM in them. At 42 degrees C, the A1-cells in larvae and DP contain a moderate amount of NSM, A2-cells of the medial group a poor, and A2-cells of the lateral group, a depleted amount of NSM, the A3-cells, however, appeared heavily loaded. Later, the NSM decreased gradually in all the subtypes of A-cells. On 15th day, at 42 degrees C the A1-cells in DP revealed depletion of NSM, the A2-cells become totally inactive and lack NSM; the A3-cells a poor amount of NSM in them. The significance of these changes at different temperature regimes is discussed.
采用副醛品红和过甲酸间苯二酚品红染色技术，研究了不同温度条件（0、7、18、37和42摄氏度）下，成熟幼虫和3月龄滞育蛹期的粘虫（鳞翅目：灯蛾科）脑内神经分泌细胞（NSCs）的情况。0摄氏度对幼虫致死，在7摄氏度时，24小时内所有A细胞亚型的神经分泌物质（NSM）数量均大幅减少；从第3天开始有活动，到第5天，A1细胞充满NSM，A2细胞中度充盈，但A3细胞未观察到变化。在0摄氏度下，滞育蛹（DP）的NSCs在48小时或冷藏后，A1细胞内有适量NSM，伴有大量大液泡，部分昆虫的细胞呈巨大形态；A2和A3细胞中度充盈NSM。在0摄氏度冷藏1周后，A1细胞几乎排空。分别置于7摄氏度和18摄氏度的滞育蛹的A1细胞，在2天内迅速排出所有先前储存的NSM，因此充盈度较差。从第3天开始，观察到NSM逐渐增加。15天后，A1细胞内有适量NSM，A3细胞内有大量NSM，内侧和外侧组的A2细胞内NSM增加。37摄氏度似乎是最适宜的温度，此时成熟幼虫和滞育蛹的所有细胞均表现出最大活性，且细胞内含有适量NSM。在42摄氏度时，幼虫和滞育蛹的A1细胞含有适量NSM，内侧组的A2细胞NSM较少，外侧组的A2细胞NSM耗尽，而A3细胞似乎充盈度很高。随后，所有A细胞亚型的NSM逐渐减少。在42摄氏度下的第15天，滞育蛹的A1细胞显示NSM耗尽，A2细胞完全无活性且缺乏NSM；A3细胞内NSM较少。文中讨论了不同温度条件下这些变化的意义。
