兔顶体蛋白酶与胰蛋白酶之间的免疫学关系。

Immunological relationships between rabbit acrosin and pancreatic trypsin.

作者信息

Dudkiewicz A B

出版信息

Immunol Commun. 1982;11(1):59-67. doi: 10.3109/08820138209050724.

DOI:10.3109/08820138209050724

Abstract

Specific anit-rabbit acrosin antibodies were prepared by immunizing female guinea pigs with rabbit acrosin. Acrosin was purified by molecular sieve and proflavin-Sepharose affinity chromatography. Inhibition studies to characterize unique antigenic determinants of acrosin compared to pancreatic trypsin indicate that anti-rabbit acrosin gamma-globulins specifically reduced the catalytic effects of acrosin but not trypsin on Azocoll. Anti-acrosin antibodies had no effect on altering the catalytic activities of either acrosin or trypsin on the substrate N-alpha-benzoyl-L-arginine ethyl ester.

摘要

通过用兔顶体酶免疫雌性豚鼠制备特异性抗兔顶体酶抗体。顶体酶通过分子筛和原黄素-琼脂糖亲和层析进行纯化。与胰蛋白酶相比，为表征顶体酶独特抗原决定簇而进行的抑制研究表明，抗兔顶体酶γ球蛋白可特异性降低顶体酶而非胰蛋白酶对偶氮酪蛋白的催化作用。抗顶体酶抗体对改变顶体酶或胰蛋白酶对底物N-α-苯甲酰-L-精氨酸乙酯的催化活性均无影响。