Johnson H M, Torres B A
Infect Immun. 1982 Jun;36(3):911-4. doi: 10.1128/iai.36.3.911-914.1982.
Immune interferon (IFN-gamma) production by mitogen-stimulated C57BL/6 mouse spleen cells required both Lyt 1+,2- and Lyt 1-,2+ lymphocytes. The Lyt 1-,2+ cells were the IFN-gamma producers, whereas the Lyt 1+,2- cells were the helpers. Interleukin 2 mediated the Lyt 1+,2- cell help. Phorbol myristic acetate (10 ng/ml) completely replaced Lyt 1+,2- helper cell or interleukin 2 requirements in IFN-gamma production. The phorbol ester exerted its helper effects by direct interaction with IFN-gamma-producing Lyt 1-,2+ cells and not through stimulation of production of interleukin 2 by Lyt 1+, 2- cells. Suppressor cell effects in IFN-gamma production were also completely abrogated by phorbol myristic acetate. The data suggest a mechanism for phorbol myristic acetate regulation of IFN-gamma production at the cellular level.
丝裂原刺激的C57BL/6小鼠脾细胞产生免疫干扰素（IFN-γ）需要Lyt 1⁺、2⁻和Lyt 1⁻、2⁺淋巴细胞。Lyt 1⁻、2⁺细胞是IFN-γ的产生者，而Lyt 1⁺、2⁻细胞是辅助细胞。白细胞介素2介导Lyt 1⁺、2⁻细胞的辅助作用。佛波醇肉豆蔻酸酯（10 ng/ml）在IFN-γ产生过程中完全取代了Lyt 1⁺、2⁻辅助细胞或白细胞介素2的需求。佛波醇酯通过与产生IFN-γ的Lyt 1⁻、2⁺细胞直接相互作用发挥其辅助作用，而不是通过刺激Lyt 1⁺、2⁻细胞产生白细胞介素2。佛波醇肉豆蔻酸酯也完全消除了IFN-γ产生过程中的抑制细胞作用。这些数据提示了佛波醇肉豆蔻酸酯在细胞水平上调节IFN-γ产生的机制。
