Edwards R G, Spackman D A, Dewdney J M
Int Arch Allergy Appl Immunol. 1982;68(4):352-7. doi: 10.1159/000233125.
The synthesis and characterisation of three novel reagents for use in the radioallergosorbent test (RAST) for the diagnosis of penicillin allergy are described. The antigenic determinants involved are the benzyl penicillanyl, thiol-linked benzyl penicillenate and thiol-linked penicillamine. These reagents, and also one specific for the benzyl penicilloyl group, have been used to evaluate the sea of subjects suspected of suffering from penicillin allergy and to explore the aetiology of the respiratory dyspnoea experienced by some workers exposed to penicillin-containing dusts. The use of these reagents, while confirming the importance of the penicilloyl or major determinant of penicillin allergy, has shown that there is heterogeneity in the IgE response of penicillin-allergic patients and some patients have IgE antibody specific for one or more of the new determinants only. These reagents will, therefore, increase diagnostic capabilities. Their use has also confirmed that the disorder induced by occupational exposure to penicillins is not primarily mediated by IgE antibody specific for allergenic determinants represented by any of the available reagents.
本文描述了三种用于青霉素过敏诊断的放射变应原吸附试验（RAST）的新型试剂的合成与表征。所涉及的抗原决定簇为苄青霉素基、硫醇连接的苄青霉素酸酯和硫醇连接的青霉胺。这些试剂，以及一种对苄青霉素酰基特异的试剂，已被用于评估疑似青霉素过敏的大量受试者，并探究一些接触含青霉素粉尘的工人所经历的呼吸性呼吸困难的病因。这些试剂的使用，在证实青霉素酰基或青霉素过敏主要决定簇的重要性的同时，表明青霉素过敏患者的IgE反应存在异质性，一些患者仅对一种或多种新的决定簇具有特异性IgE抗体。因此，这些试剂将提高诊断能力。它们的使用还证实，职业性接触青霉素所诱发的病症并非主要由针对任何现有试剂所代表的变应原决定簇的特异性IgE抗体介导。
