Grossgebauer K, Rolly H
Microsc Acta. 1982 May;86(1):1-11.
The newer DNA-binding fluorochromes DAPI (4',6-diamidino-2-phenylindole) and fluorochrome 33258 H (Hoechst) (2-[2-(4-hydroxy-phenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)-benzimidazole . 3 HCl) proved useful in identifying genital strains of Chlamydia trachomatis in McCoy cells. For practical purposes, e.g. to analyse patient specimens, we recommend this technique using the fluorochromes at pH 2.0 in a final concentration of 3 micrograms/ml and non-replicative McCoy cells after fixation with alcohol-acetic acid. The application of the fluorochrome technique in demonstrating Chlamydia trachomatis-infections is recommendable because it is a) simple to perform, b) a rapid procedure, c) it corresponded well with the Giemsa staining in identifying mature inclusions, and d) it facilitates the detection of the RNA-rich early stages of chlamydial growth. For this reasons the fluorochrome technique with DAPI or 33258 H at pH 2.0 can be considered a highly specific and sensitive method for identifying Chlamydia trachomatis.
新型DNA结合荧光染料4',6-二脒基-2-苯基吲哚（DAPI）和荧光染料33258 H（Hoechst）（2-[2-(4-羟基苯基)-6-苯并咪唑基]-6-(1-甲基-4-哌嗪基)-苯并咪唑·3盐酸盐）已被证明可用于在McCoy细胞中鉴定沙眼衣原体的生殖道菌株。出于实际目的，例如分析患者标本，我们推荐在pH 2.0条件下使用终浓度为3微克/毫升的荧光染料以及经酒精-乙酸固定后的非复制性McCoy细胞的这项技术。荧光染料技术在证明沙眼衣原体感染方面的应用是值得推荐的，因为它：a）操作简单；b）程序快速；c）在鉴定成熟包涵体方面与吉姆萨染色结果吻合良好；d）有助于检测衣原体生长中富含RNA的早期阶段。基于这些原因，在pH 2.0条件下使用DAPI或33258 H的荧光染料技术可被视为鉴定沙眼衣原体的一种高度特异性和敏感性的方法。
