Nishikawa A, Shinoda T, Fukazawa Y
Mol Immunol. 1982 Mar;19(3):367-73. doi: 10.1016/0161-5890(82)90202-4.
We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysis of the mannan gave a large amount of mannooctaose. We have examined the inhibition by these oligosaccharides of the precipitin reaction between anti-Candida krusei serum and homologous mannan, and found that the mannooctaose was the most effective inhibitor. Results obtained by proton magnetic resonance spectroscopy, methylation analysis, and other structural studies of Candida krusei mannan, suggest that the octasaccharide possesses six alpha (1-2) linkages and one alpha (1-6) linkage located in the middle of the chain, and that this mannooctaose may be responsible for the specificity of Candida krusei cell-wall mannan.
我们研究了克鲁斯念珠菌细胞壁甘露聚糖抗原决定簇的化学结构。用碱提取并纯化得到的铜络合物形式的甘露聚糖进行乙酰解反应，得到了五种低聚糖（从单糖到五糖）和少量的八糖。甘露聚糖的部分乙酰解反应产生了大量的甘露八糖。我们检测了这些低聚糖对抗克鲁斯念珠菌血清与同源甘露聚糖之间沉淀反应的抑制作用，发现甘露八糖是最有效的抑制剂。通过质子磁共振光谱、甲基化分析以及对克鲁斯念珠菌甘露聚糖的其他结构研究得到的结果表明，该八糖具有六个α（1-2）键和一个位于链中间的α（1-6）键，并且这种甘露八糖可能是克鲁斯念珠菌细胞壁甘露聚糖特异性的原因。
