Funayama M, Nishikawa A, Shinoda T, Suzuki M, Fukazawa Y
Microbiol Immunol. 1984;28(12):1359-71. doi: 10.1111/j.1348-0421.1984.tb00794.x.
We examined the antigenic relationship between Candida parapsilosis and C. albicans serotype B with respect to antigenic factors 13 and 13b, specific for the former species and common to both species, respectively. Acetolysis of C. albicans serotype B cell-wall mannan gave six oligosaccharides. Their chemical structure was determined by 1H-nuclear magnetic resonance (NMR) spectroscopy, methylation analysis, and partial acid hydrolysis. The structure of the hexasaccharide derived from C. albicans serotype B mannan was alpha-D-Manp-(1-2)-alpha-D-Manp-(1-3)-alpha-D-Manp-(1- 2)-alpha-D-Manp-(1-2)- alpha-D-Manp-(1-2)-D-Man (M6) which is identical to that from C. parapsilosis mannan. Inhibition of two precipitin reaction systems (anti-C. albicans serotype B serum and anti-C. parapsilosis serum to the respective homologous mannan), by oligosaccharides from homologous and heterologous mannans indicated that M6 from either C. albicans serotype B or C. parapsilosis was the most effective inhibitor. Moreover inhibition of the agglutination reaction between factor serum containing anti-factors 13 and 13b and C. albicans serotype B or C. parapsilosis cells by oligosaccharides from both mannans also indicated that the M6s were the most effective inhibitors. These results suggest that the M6s derived from the two species are identical in their chemical structure, although the structures of the whole mannans of the two species are not identical as demonstrated by gel diffusion precipitation patterns, and that M6s may be involved in the specificities of antigenic factors 13 and 13b. The amount of M6 is larger in C. parapsilosis cell-wall mannan, suggesting that high repeating frequency of M6 fragment may induce the antibody specific for C. parapsilosis.
我们针对分别对近平滑念珠菌特异以及二者共有的抗原因子13和13b，研究了近平滑念珠菌与白念珠菌B血清型之间的抗原关系。对白念珠菌B血清型细胞壁甘露聚糖进行乙酰解得到了六种低聚糖。通过1H-核磁共振（NMR）光谱、甲基化分析和部分酸水解确定了它们的化学结构。源自白念珠菌B血清型甘露聚糖的六糖结构为α-D-甘露糖基-(1-2)-α-D-甘露糖基-(1-3)-α-D-甘露糖基-(1-2)-α-D-甘露糖基-(1-2)-α-D-甘露糖基-(1-2)-D-甘露糖（M6），这与近平滑念珠菌甘露聚糖的结构相同。同源和异源甘露聚糖的低聚糖对两个沉淀反应系统（抗白念珠菌B血清和抗近平滑念珠菌血清对各自同源甘露聚糖的反应）的抑制作用表明，来自白念珠菌B血清型或近平滑念珠菌的M6是最有效的抑制剂。此外，两种甘露聚糖的低聚糖对含有抗因子13和13b的因子血清与白念珠菌B血清型或近平滑念珠菌细胞之间的凝集反应的抑制作用也表明，M6是最有效的抑制剂。这些结果表明，尽管通过凝胶扩散沉淀模式证明这两个物种的整个甘露聚糖结构不同，但源自这两个物种的M6在化学结构上是相同的，并且M6可能与抗原因子1,3和13b的特异性有关。近平滑念珠菌细胞壁甘露聚糖中M6的含量更高，这表明M6片段的高重复频率可能诱导产生针对近平滑念珠菌的抗体。
