Sauvalle A M, Prigent J R, Izard J Y
Acta Cytol. 1982 May-Jun;26(3):371-5.
A new method of comparing light microscopy and scanning electron microscopy in the study of small cells, such as spermatozoa, that must be examined under oil immersion is described. A grid is etched on the corner of a microscope glass slide, and its inner edges are incised. Its surface area is calculated as a function f the chamber of the critical-point drying apparatus. This method dispenses with the need for any special coverslip and enables the cells to be observed under oil immersion.
描述了一种在研究如精子等必须在油浸条件下检查的小细胞时，比较光学显微镜和扫描电子显微镜的新方法。在显微镜载玻片的一角蚀刻一个网格，并切割其内部边缘。其表面积根据临界点干燥设备的腔室进行计算。该方法无需任何特殊盖玻片，可使细胞在油浸条件下被观察。
