Katoh Y
No To Shinkei. 1982 May;34(5):479-83.
The intracytoplasmic inclusion bodies were found to be intensely stained in the normal nerve cells by the modified Holmes method (Katoh and Shimizu, 1982b). In the present study we used ddY mouse under normal and experimental conditions and stained locus coeruleus region, where the inclusion bodies are remarkably rich in number. The inclusion bodies stained by Holmes method were round in shape and 1--3 micrometers in size and were present singly or 2 in one cell. Total number of these bodies amounted to 1569 +/- 166 in the unilateral locus coeruleus in both sexes. Experimental conditions, such as dehydration for 3--6 days, fasting for 3--6 days, dehydration plus fasting for 3--6 days and reserpine (5 mg/Kg) administration markedly decreased the number of the bodies. However castration (10 to 30 days after operation) and vasopressin (2 IU each for 13 days) produced no changes. Nialamide (300 mg/Kg each for 3 days) caused slight increase. In the ontogenetic development the bodies already appeared in the late fetal life.
采用改良的霍姆斯方法（加藤和清水，1982b）发现，正常神经细胞中的胞质内包涵体被强烈染色。在本研究中，我们在正常和实验条件下使用ddY小鼠，并对蓝斑区域进行染色，该区域的包涵体数量非常丰富。用霍姆斯方法染色的包涵体呈圆形，大小为1 - 3微米，单个或两个存在于一个细胞中。在两性的单侧蓝斑中，这些包涵体的总数为1569±166个。实验条件，如脱水3 - 6天、禁食3 - 6天、脱水加禁食3 - 6天以及给予利血平（5毫克/千克），均显著减少了包涵体的数量。然而，去势（术后10至30天）和血管加压素（每次2国际单位，共13天）并未产生变化。尼亚酰胺（每次300毫克/千克，共3天）导致轻微增加。在个体发育过程中，这些包涵体在胎儿后期就已出现。
