Paulsen H, Jacquinet J C, Rust W
Carbohydr Res. 1982 Jun 16;104(2):195-219. doi: 10.1016/s0008-6215(00)82583-5.
The hapten of the T-antigen was synthesized with a peptide-like amide-spacer as 2-(4-methoxycarbonylbutanecarboxamido)ethyl 2-acetamido-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside and coupled with serum albumin to give a synthetic antigen. Other O-beta-D-galactopyranosyl haptens, 2-(4-methoxycarbonylbutanecarboxamido)ethyl 2-acetamido-2-deoxy-4-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside, O-beta-D-galactopyranosyl-(1 leads to 3)-O-[beta-D-galactopyranosyl-(1 leads to 4)]-2-acetamido-2-deoxy-alpha-D-galactopyranoside, and 2-acetamido-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside, the last compound being the determinant of the Lewis Lec antigen, were also synthesized.
T抗原的半抗原是用一种类似肽的酰胺间隔基合成的，即2-(4-甲氧羰基丁烷甲酰胺基)乙基2-乙酰氨基-2-脱氧-3-O-β-D-吡喃半乳糖基-α-D-吡喃半乳糖苷，并与血清白蛋白偶联得到一种合成抗原。还合成了其他O-β-D-吡喃半乳糖基半抗原，即2-(4-甲氧羰基丁烷甲酰胺基)乙基2-乙酰氨基-2-脱氧-4-O-β-D-吡喃半乳糖基-α-D-吡喃半乳糖苷、O-β-D-吡喃半乳糖基-(1→3)-O-[β-D-吡喃半乳糖基-(1→4)]-2-乙酰氨基-2-脱氧-α-D-吡喃半乳糖苷，以及2-乙酰氨基-2-脱氧-3-O-β-D-吡喃半乳糖基-α-D-吡喃葡萄糖苷，最后一种化合物是Lewis Lec抗原的决定簇。
