Handman E, Hocking R E
Infect Immun. 1982 Jul;37(1):28-33. doi: 10.1128/iai.37.1.28-33.1982.
The fusion of NS-1 myeloma cells with spleen cells from mice chronically infected with Leishmania tropica resulted in nine clones of hybridomas producing monospecific antibodies to membrane antigens of L. tropica. One of the antibodies (L-5-85) bound specifically to the promastigote form of the parasite, and the remaining eight recognized antigens shared by the promastigote and amastigote of L. tropica. Four of the antibodies (L-5-16, L-5-34, L-5-44, and L-2-3F11) detected parasite antigens on the surface of L. tropica-infected macrophages. Common antigens shared by L. tropica, L. mexicana, and L. donovani were identified as well as one antigen apparent on most Leishmania spp. and present also in Crithidia fasciculata. Two monoclonal antibodies (L-5-27 and L-5-41) were found to bind only to strains of L. tropica from simple cutaneous leishmaniasis. A special property shared by these two antibodies was the inhibition of parasite growth in macrophages in vitro.
将热带利什曼原虫慢性感染小鼠的脾细胞与NS-1骨髓瘤细胞融合，产生了9个杂交瘤克隆，这些克隆产生针对热带利什曼原虫膜抗原的单特异性抗体。其中一种抗体（L-5-85）特异性结合该寄生虫的前鞭毛体形式，其余8种识别热带利什曼原虫前鞭毛体和无鞭毛体共有的抗原。4种抗体（L-5-16、L-5-34、L-5-44和L-2-3F11）检测到热带利什曼原虫感染的巨噬细胞表面的寄生虫抗原。鉴定出了热带利什曼原虫、墨西哥利什曼原虫和杜氏利什曼原虫共有的抗原，以及在大多数利什曼原虫属中明显存在且在纤细短膜虫中也存在的一种抗原。发现两种单克隆抗体（L-5-27和L-5-41）仅与单纯皮肤利什曼病的热带利什曼原虫菌株结合。这两种抗体共有的一个特殊特性是在体外抑制巨噬细胞中寄生虫的生长。
