Hockmeyer W T, Walters D, Gore R W, Williams J S, Fortier A H, Nacy C A
J Immunol. 1984 Jun;132(6):3120-5.
Resident peritoneal macrophages were exposed to amastigotes of Leishmania donovani and L. tropica and treated with soluble products of antigen or mitogen-stimulated lymphocytes (lymphokines, LK). Macrophages infected with L. donovani were responsive to lower concentrations of LK for elimination of this parasite than cells infected with L. tropica, and the disappearance of L. donovani amastigotes from activated macrophages preceded that of L. tropica by at least 24 hr. That the differential elimination of amastigotes from LK-treated macrophages may reflect different intracellular killing mechanisms for the two parasites is supported by studies with macrophages activated in vivo; macrophages from BCG-treated C3H/HeJ mice that were incapable of eliminating intracellular L. tropica killed amastigotes of L. donovani. Whether the effector mechanism(s) for intracellular destruction of the two Leishmania are qualitatively or only quantitatively different is as yet unknown.
将腹腔巨噬细胞暴露于杜氏利什曼原虫和热带利什曼原虫的无鞭毛体，并使用抗原或丝裂原刺激淋巴细胞的可溶性产物（淋巴因子，LK）进行处理。与感染热带利什曼原虫的细胞相比，感染杜氏利什曼原虫的巨噬细胞对较低浓度的LK有反应以消除这种寄生虫，并且活化巨噬细胞中杜氏利什曼原虫无鞭毛体的消失比热带利什曼原虫至少提前24小时。体内活化巨噬细胞的研究支持，从经LK处理的巨噬细胞中差异消除无鞭毛体可能反映了两种寄生虫不同的细胞内杀伤机制；来自卡介苗处理的C3H/HeJ小鼠的巨噬细胞无法消除细胞内的热带利什曼原虫，但能杀死杜氏利什曼原虫的无鞭毛体。两种利什曼原虫细胞内破坏的效应机制在性质上还是仅在数量上不同，目前尚不清楚。
