Furmanski P, Loeckner C P, Longley C, Larson L J, Rich M A
Cancer Res. 1976 Nov;36(11 Pt 1):4001-7.
Subviral cores have been prepared from the oncornavirus-like particle found in human milks with the use of phospholipase C and ether or Sterox SL. The major protein of these cores has a molecular weight of 27,000 daltons, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein is found in the core fractions of reverse transcriptase-positive milks and is absent in negative milks. It is distributed in sucrose gradients only in those fractions containing cores and reverse transcriptase activity. The major core protein of the human milk oncornavirus-like particle is electrophoretically identical to the major core protein of the mouse mammary tumor virus.
已使用磷脂酶C和乙醚或Sterox SL从人乳中发现的类肿瘤病毒颗粒制备了亚病毒核心。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，这些核心的主要蛋白质分子量为27,000道尔顿。这种蛋白质存在于逆转录酶阳性乳汁的核心部分，而在阴性乳汁中不存在。它仅分布在含有核心和逆转录酶活性的蔗糖梯度部分中。人乳类肿瘤病毒颗粒的主要核心蛋白在电泳上与小鼠乳腺肿瘤病毒的主要核心蛋白相同。
