Kucera J
Histochemistry. 1982;74(4):493-503. doi: 10.1007/BF00496663.
Cat muscle spindles were studied histochemically in serial transverse sections of the tenuissimus muscle stained for myofibrillar ATPase, cholinesterase or NADH-tetrazolium reductase. The terminal sites of the primary and secondary axons on intrafusal muscle fibers could be demonstrated due to their high NADH-TR activity. This sensory NADH-TR reactivity at the equator and in the juxtaequatorial regions disappeared following spindle chronic de-afferentation, but not after de-efferentation. Spindle poles that carried both primary and secondary sensory endings had a longer periaxial fluid space than poles with primary endings only, and their motor innervation, as determined by staining for ChE, was positioned at the greater distance from the equator. Some of the secondary endings occurred in intrafusal regions that displayed surface fiber ChE activity. The histochemical reaction for NADH-TR represents a simple, rapid and reliable method for studies of the distribution of sensory nerve terminals in the spindle.
在对细肌进行连续横切片的组织化学研究中，对猫的肌梭进行了研究，这些切片用肌原纤维ATP酶、胆碱酯酶或NADH-四唑还原酶进行染色。由于初级和次级轴突在梭内肌纤维上的终末部位具有高NADH-TR活性，因此可以显示出来。在梭慢性去传入后，赤道和近赤道区域的这种感觉NADH-TR反应性消失，但去传出后则不会消失。携带初级和次级感觉末梢的梭极比仅带有初级末梢的梭极具有更长的轴周液空间，并且通过ChE染色确定，它们的运动神经支配位于离赤道更远的位置。一些次级末梢出现在显示表面纤维ChE活性的梭内区域。NADH-TR的组织化学反应是研究梭内感觉神经末梢分布的一种简单、快速且可靠的方法。
