Allan J, Smith B J, Dunn B, Bustin M
J Biol Chem. 1982 Sep 25;257(18):10533-5.
Antibodies to the folding domain (residues 22-100) of histone H5 were elicited in rabbits. Analysis of the specificity of these antibodies by enzyme-linked immunoassay and by diazobenzyloxymethyl cellulose transfer techniques revealed that the antibody cross-reacts strongly with intact H5 and histones H1(0)a and H1(0)b purified from ox liver but not with the four core calf thymus, or with high mobility group proteins. We conclude that the globular region of H5 is serologically homologous to that of H1 degrees and suggest that possible functional similarities between the two proteins reside in this region.
在兔子体内产生了针对组蛋白H5折叠结构域（第22至100位氨基酸残基）的抗体。通过酶联免疫测定法和重氮苄氧基甲基纤维素转移技术对这些抗体的特异性进行分析，结果显示该抗体与完整的H5以及从牛肝中纯化得到的组蛋白H1(0)a和H1(0)b发生强烈交叉反应，但与小牛胸腺的四种核心组蛋白或高迁移率族蛋白不发生交叉反应。我们得出结论，H5的球状区域在血清学上与H1°的球状区域同源，并表明这两种蛋白质之间可能存在的功能相似性存在于该区域。
