通过放射免疫测定法测定鱼类有核红细胞中的组蛋白H5。
Histone H5 in nucleated erythrocytes of fish as determined by radioimmunoassay.
作者信息
Goetz G, Esmailzadeh A K, Huang P C
出版信息
Biochim Biophys Acta. 1978 Jan 26;517(1):236-45. doi: 10.1016/0005-2787(78)90051-5.
Tissue-specific histone H5 in the nucleated erythrocytes of dogfish, scup, skate, tautog, sea robin and toad fish was studied. The presence of this histone was inferred by its electrophoretic mocility on polyacrylamide gels containing either acid-urea or sodium dodecyl sulfate. By radioimmunoprecipitation assays, cross reaction was observed between fish histones and an anti-H5chicken antibody. The antibody was specific to chicken histone H5; purified chicken histone H1 and calf thymus total histones did not cross react. It is concluded that fish histone H5 shares common antigenic determinants with the chicken H5 histone.
对狗鲨、鲷鱼、鳐鱼、黑鳍鲔、海鲂和蟾鱼有核红细胞中的组织特异性组蛋白H5进行了研究。通过其在含有酸尿素或十二烷基硫酸钠的聚丙烯酰胺凝胶上的电泳迁移率推断出这种组蛋白的存在。通过放射免疫沉淀试验，观察到鱼类组蛋白与抗H5鸡抗体之间的交叉反应。该抗体对鸡组蛋白H5具有特异性；纯化的鸡组蛋白H1和小牛胸腺总组蛋白没有交叉反应。得出的结论是，鱼类组蛋白H5与鸡H5组蛋白具有共同的抗原决定簇。
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