Warburton M J, Mitchell D, Ormerod E J, Rudland P
J Histochem Cytochem. 1982 Jul;30(7):667-76. doi: 10.1177/30.7.6179984.
Using antisera to specific proteins, the localization of the rat mammary parenchymal cells (both epithelial and myoepithelial), the basement membrane, and connective tissue components has been studied during the four physiological stages of the adult rat mammary gland, viz. resting, pregnant, lactating, and involuting glands. Antisera to myosin and prekeratin were used to localize myoepithelial cells, antisera to rat milk fat globule membrane for epithelial cells, antisera to laminin and type IV collagen to delineate the basement membrane and antisera to type I collagen and fibronectin as markers for connective tissue. In the resting, virgin mammary gland, myoepithelial cells appear to form a continuous layer around the epithelial cells and are in turn surrounded by a continuous basement membrane. Antiserum to fibronectin does not delineate the basement membrane in the resting gland. The ductal system is surrounded by connective tissue. Only the basal or myoepithelial cells in the terminal end buds of neonatal animals demonstrate cytoplasmic staining for basement membrane proteins, indicating active synthesis of these proteins during this period. In the secretory alveoli of the lactating rat, the myoepithelial cells no longer appear to form a continuous layer beneath the epithelial cells and in many areas the epithelial cells appear to be in contact with the basement membrane. The basement membrane in the lactating gland is still continuous around the ducts and alveoli. In the lactating gland, fibronectin appears to be located in the basement membrane region in addition to being a component of the stroma. During involution, the alveoli collapse, and appear to be in a state of dissolution. The basement membrane is thicker and is occasionally incomplete, as also are the basket-like myoepithelial structures. Basement membrane components can also be demonstrated throughout the collapsed alveoli.
利用针对特定蛋白质的抗血清，对成年大鼠乳腺在四个生理阶段，即静止期、孕期、哺乳期和退化期的乳腺实质细胞（上皮细胞和肌上皮细胞）、基底膜和结缔组织成分的定位进行了研究。使用抗肌球蛋白和前角蛋白的抗血清来定位肌上皮细胞，用抗大鼠乳脂肪球膜的抗血清来定位上皮细胞，用抗层粘连蛋白和IV型胶原的抗血清来描绘基底膜，并用抗I型胶原和纤连蛋白的抗血清作为结缔组织的标志物。在静止期的处女乳腺中，肌上皮细胞似乎在上皮细胞周围形成连续的一层，而其自身又被连续的基底膜所包围。抗纤连蛋白的抗血清在静止期的腺体中不能描绘出基底膜。导管系统被结缔组织所包围。只有新生动物终末芽中的基底或肌上皮细胞显示出基底膜蛋白的细胞质染色，表明在此期间这些蛋白有活跃的合成。在泌乳大鼠的分泌性肺泡中，肌上皮细胞似乎不再在上皮细胞下方形成连续的一层，在许多区域上皮细胞似乎与基底膜接触。泌乳期腺体的基底膜在导管和肺泡周围仍然是连续的。在泌乳期腺体中，纤连蛋白除了是间质的组成成分外，似乎还位于基底膜区域。在退化期，肺泡塌陷，似乎处于溶解状态。基底膜变厚，偶尔不完整，篮状的肌上皮结构也是如此。基底膜成分在整个塌陷的肺泡中也能被显示出来。
