Warburton M J, Monaghan P, Ferns S A, Rudland P S, Perusinghe N, Chung A E
Exp Cell Res. 1984 May;152(1):240-54. doi: 10.1016/0014-4827(84)90249-0.
Entactin, a sulfated glycoprotein with a molecular weight (MW) of about 150 kD, is present in vascular basement membranes and in the interstitial connective tissue of the mammary glands of virgin rats. It does not appear to be present in the basement membrane surrounding the mammary ductal system. However, in lactating mammary glands entactin is also present in the basement membrane region surrounding the secretory alveoli. Ultrastructural localisation of entactin reveals that it is present on the basal surface of epithelial cells, with patchy staining in the lamina lucida and lamina densa. Entactin also appears to be associated with interstitial collagen fibres. Mammary fibroblastic cells in culture are able to produce entactin, whereas mammary epithelial and myoepithelial cells, which synthesise the basement membrane proteins laminin and type IV collagen, fail to synthesise entactin.
巢蛋白是一种分子量约为150kD的硫酸化糖蛋白，存在于血管基底膜和未生育大鼠乳腺的间质结缔组织中。它似乎不存在于乳腺导管系统周围的基底膜中。然而，在泌乳期乳腺中，巢蛋白也存在于分泌性肺泡周围的基底膜区域。巢蛋白的超微结构定位显示，它存在于上皮细胞的基底表面，在透明层和致密层中有斑点状染色。巢蛋白似乎也与间质胶原纤维有关。培养中的乳腺成纤维细胞能够产生巢蛋白，而合成基底膜蛋白层粘连蛋白和IV型胶原的乳腺上皮细胞和肌上皮细胞则不能合成巢蛋白。
