Buell S J
J Neuropathol Exp Neurol. 1982 Sep;41(5):500-7. doi: 10.1097/00005072-198209000-00003.
Comparisons were made between the results of applying the Golgi-Cox and rapid Golgi techniques to human brain tissue obtained at autopsy. Adjacent blocks of hippocampal formation and precentral gyrus from nine cases were prepared by the two methods. The cases ranged in age from 39 to 99 years, had postmortem times for sampling ranging from 6 to 28 hours (h) and included cases of dementia of the Alzheimer type. Without exception, the methods produced very different results. The Golgi-Cox method resulted in impregnation of many neurons with rich dendritic plexuses and normal overall appearance. Occasional cells appeared grossly atrophic with irregular somata and apparent loss of apical and basilar dendritic segments. With the rapid Golgi method, the vast majority of impregnated neurons had rich dendritic plexuses or were otherwise normal in appearance. Thus, the rapid Golgi method appears to be highly sensitive to postmortem delay or other factors which accompany studies involving human brain tissues obtained at autopsy. The Golgi-Cox method appears to be relatively insensitive to such factors.
对应用高尔基-考克斯法和快速高尔基法处理尸检获得的人脑组织的结果进行了比较。用这两种方法对9例相邻的海马结构和中央前回组织块进行了处理。这些病例年龄在39岁至99岁之间，死后取样时间为6至28小时，包括阿尔茨海默型痴呆病例。毫无例外，这两种方法产生了非常不同的结果。高尔基-考克斯法使许多神经元被浸染，树突丛丰富，整体外观正常。偶尔有细胞出现明显萎缩，胞体不规则，顶树突和基底树突节段明显缺失。使用快速高尔基法时，绝大多数被浸染的神经元树突丛丰富，或外观上其他方面正常。因此，快速高尔基法似乎对死后延迟或其他伴随涉及尸检获得的人脑组织研究的因素高度敏感。高尔基-考克斯法似乎对这些因素相对不敏感。
