Taylor A N, McIntosh J E, Bourdeau J E
Kidney Int. 1982 May;21(5):765-73. doi: 10.1038/ki.1982.95.
Vitamin D-dependent calcium-binding protein (CaBP) was localized in tissue sections of kidneys from rabbits, rats, and chicks using antiserum specific for chick intestinal CaBP. In rabbit kidney, CaBP was present in all cells of the distal convoluted tubule and most cells of the connecting tubule. Fewer, but still a majority, of the cells of cortical collecting ducts contained CaBP. The intensity of immunochemical staining and the number of stained cells decreased markedly in medullary collecting ducts, and only a few collecting duct cells contained CaBP at the junction of the inner and outer medulla. In the rat kidney, CaBP was present in all distal convoluted tubule cells, but the immunochemical staining was less intense than in the rabbit. The protein also was found in most connecting tubule cells of the rat; however, only a few collecting duct cells in the superficial corte of the rat contained CaBP. CaBP was essentially absent from mid- to deep-cortical collecting duct cells, while a very few collecting duct cells always contained CaBP at the junction of the inner and outer stripes of the outer medulla. In the chick, CaBP was present in distal convoluted tubule cells as the distal convoluted tubule coursed adjacent to the central vein. CaBP was absent from chick collecting duct cells. In all three species CaBP was not detected in the other portions of the nephron.
使用针对鸡肠钙结合蛋白（CaBP）的抗血清，将维生素D依赖性钙结合蛋白（CaBP）定位在兔、大鼠和鸡肾脏的组织切片中。在兔肾中，CaBP存在于远曲小管的所有细胞以及连接小管的大多数细胞中。皮质集合管中含有CaBP的细胞较少，但仍占多数。髓质集合管中免疫化学染色强度和染色细胞数量明显降低，在内髓和外髓交界处只有少数集合管细胞含有CaBP。在大鼠肾脏中，CaBP存在于所有远曲小管细胞中，但免疫化学染色强度低于兔肾。该蛋白在大鼠的大多数连接小管细胞中也有发现；然而，在大鼠浅皮质中只有少数集合管细胞含有CaBP。皮质中至深层的集合管细胞基本没有CaBP，而在外髓内带和外带交界处总是有极少数集合管细胞含有CaBP。在鸡中，当远曲小管靠近中央静脉走行时，CaBP存在于远曲小管细胞中。鸡的集合管细胞中没有CaBP。在所有这三个物种中，在肾单位的其他部分未检测到CaBP。
