大鼠腮腺淀粉酶：外分泌蛋白随年龄增长发生变化的证据。

Rat parotid gland amylase: evidence for alterations in an exocrine protein with increased age.

作者信息

Baum B J, Levine R L, Kuyatt B L, Sogin D B

出版信息

Mech Ageing Dev. 1982 May;19(1):27-35. doi: 10.1016/0047-6374(82)90047-1.

Abstract

The content of alpha-amylase, the major exocrine secretory protein from rat parotid glands, was studied in young adult and aged rat tissue. alpha-Amylase protein was determined with an enzyme-linked immunosorbent assay. This employed antisera, produced against alpha-amylase purified from young adult rats, but which recognized and precipitated alpha-amylase enzyme activity equally well from both age groups. The parotid gland content of alpha-amylase was reduced about 50% in aged rats. Furthermore, the percentage of total gland protein which was alpha-amylase was decreased about 40% in aged animals. The data suggest that a somewhat specific alteration in alpha-amylase production (synthesis and/or degradation) occurs in parotid glands from aged rats. In addition, alpha-amylase functional activity was followed. The specific enzyme activity (U amylase activity per mg immunoreactive amylase) was about 35% higher in extracts from aged rat parotid glands compared to that of young adult glands.

摘要

在年轻成年大鼠和老年大鼠组织中，研究了大鼠腮腺主要外分泌分泌蛋白α-淀粉酶的含量。采用酶联免疫吸附测定法测定α-淀粉酶蛋白。该方法使用针对从年轻成年大鼠纯化的α-淀粉酶产生的抗血清，但该抗血清能同等良好地识别和沉淀两个年龄组的α-淀粉酶酶活性。老年大鼠腮腺中α-淀粉酶的含量降低了约50%。此外，在老年动物中，作为α-淀粉酶的总腺蛋白百分比降低了约40%。数据表明，老年大鼠腮腺中α-淀粉酶产生（合成和/或降解）发生了某种特定改变。此外，还跟踪了α-淀粉酶的功能活性。与年轻成年腺体相比，老年大鼠腮腺提取物中的比酶活性（每毫克免疫反应性淀粉酶的淀粉酶活性单位）高约35%。